Stability Studies of Immobilized Saccharomyces Cerevisiae in Calcium Alginate and Carrageenan Beads
Abstract
Currently the resources for fossil fuels are depleting together with increase in fuel prices. This has urged the need for cheaper alternative fuels especially biofuels. The production of the most common liquid biofuel which is bioethanol using immobilized yeast cells is an approach taken to increase its demand in the world’s market. There are various methods for the immobilization of yeast cells; however before they can be applied in the industry the stability of the immobilization technology must be investigated. This research aims to study the stabilities of immobilized S. cerevisiae in calcium alginate and carrageenan beads for bioethanol production. The S. cerevisiae was immobilized in calcium alginate and carrageenan beads using entrapment method. Next, screening for the optimal concentration of sodium alginate and semi refined carrageenan matrices were determined by employing fermentation and bioethanol quantification using GC-MS. Concentrations of 2% (w/v) calcium alginate and 2% (w/v) semi refined carrageenan beads were identified to produce the highest bioethanol yield which were 0.286 g/mL and 0.065 g/mL respectively. The two beads were then chosen to be tested in various stability studies with respect to bioethanol production such as storage stability, reusability, pH, thermaland permeability test. It was found out that a concentration of 2% (w/v) calcium alginate beads were more stable as immobilization matrix for S. cerevisiae as compared to 2% (w/v) semi refined carrageenan.
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Copyright & License
All Research Plus Journals (RPJ) publish open access articles under the terms of the Creative Commons Attribution (CC BY-SA 4.0) https://creativecommons.org/licenses/by-sa/4.0/ License which permits use, distribution and reproduction in any medium, provided the original work is properly cited & ShareAlike terms followed.
Copyright on any research article in a journal published by a RPJ is retained by the author(s). Authors grant RPJ a license to publish the article and identify itself as the original publisher. Upon author(s) by giving permission to RPJ either via RPJ journal portal or other channel to publish their research work in RPJ agrees to all the terms and conditions of https://creativecommons.org/licenses/by-sa/4.0/ License and terms & condition set by RPJ.
3rd party copyright
It is the responsibility of author(s) to secure all necessary copyright permissions for the use of 3rd-party materials in their manuscript.
Disclaimer
Research Plus Journals Open Access articles posted to repositories or websites are without warranty from RPJ of any kind, either express or implied, including, but not limited to, warranties of merchantability, fitness for a particular purpose, or non-infringement. To the fullest extent permitted by law RPJ disclaims all liability for any loss or damage arising out of, or in connection, with the use of or inability to use the content.
